5 Steps to Grow Mushrooms in a Monotub

Discover the joy of growing your mushrooms at home with the Monotub Mushroom Grow Kit from Myterra Labs. In this easy-to-follow guide, we'll walk you through the process of cultivating mushrooms in a monotub, providing you with the essential steps and tips for a successful harvest. Let's embark on this exciting journey together!

What is a Monotub?

A monotub is a simple container used for horizontal mushroom cultivation, enclosing the growing surface in a controlled environment. Myterra Labs offers the Max Yield Bin as part of their monotub mushroom grow kit, featuring pre-drilled holes for optimal airflow and a set-and-forget design. The V2 tapered lid enhances your growing experience by preventing condensation droplets from falling on your mushrooms. Explore the Monotub Mushroom Grow Kit here.

What You'll Need

Myterra Labs' Monotub Mushroom Grow Kit has everything you need to get started, including substrate and grain bags. If you have your monotub, consider the Re-Up bundle containing manure-free vegan substrate and organic rye berries here.

Additional supplies include:

  • Your favorite mushroom strain in liquid culture or on an agar plate
  • 70% isopropyl alcohol in a spray bottle
  • Latex gloves (recommended)
  • Micropore tape (included in Myterra Labs Mushroom Monotub Kit)

Step 1: Inoculate Grain

Inoculation involves adding a living culture to a growing medium. Myterra Labs provides two methods:

a) Liquid Culture:

  • Wipe the grain bag with isopropyl alcohol.
  • Attach the injection port and wipe with alcohol.
  • Inject culture, place the bag on a shelf out of direct sunlight.

b) Agar Culture:

  • Wipe the bag outside with isopropyl alcohol.
  • Cut the top off in a sterile environment.
  • Transfer agar wedges into the open grain bag using a sterilized scalpel.
  • Seal the bag and shake to distribute agar pieces.

Step 2: Grain Spawn

Shake the bag when you see healthy white mycelium growth after 7 days. Once fully colonized (1-2 weeks), you've successfully created grain spawn. Monitor for contamination.

Step 3: Prepare Your Monotub

Cover holes in the lid and base with micropore tape. Spray and wipe the bin, liner, spawn, and substrate bags with alcohol.

Step 4: Inoculate Substrate

In a clean area, cut open bags, pour spawn and substrate into the bin, mix thoroughly, and press down lightly. Replace the lid and keep the container out of direct sunlight at room temperature.

Step 5: Set-And-Forget

Over the next weeks, watch the mycelium spread. Micropore tape allows air exchange while retaining humidity. Open the bin occasionally once the surface has white mycelium poking through.

Harvest approximately 3 weeks after inoculation when mushrooms are ready. Twist the stem gently to harvest, ensuring the veil is intact. Clean tools to prevent contamination.

Harvest Tip: Harvest mushrooms when the veils are intact, avoiding spore release.


Growing mushrooms in a monotub is a rewarding and accessible journey. Myterra Labs simplifies the process with quality kits, making it easy for anyone to enjoy fresh, homegrown mushrooms. Start your mushroom cultivation adventure today!